RESUMO
The goal of this study was to evaluate different methods of cultivating human bone cells. Five periosteal and bone specimens obtained from the human jaw were divided into small pieces in the laboratory. After the addition of trypsin and collagenase enzymes and releasing of cells, three primary periostal, endosteal, and bony chips cells were prepared. After passing the required time for the growth of specimens, lamella were prepared and stained with alkaline phosphatase [ALP] in order to determine ALP positive cells. Mean time of cellular growth was 23 days. Human bone cells have the capability of being cultured under special sterile laboratory conditions and three dimensional culturing of them can be used for reconstruction of maxillofacial region defects